Journal: Developmental biology

Article Title: Myoblast alpha v beta3 integrin levels are controlled by transcriptional regulation of expression of the beta3 subunit and down-regulation of beta3 subunit expression is required for skeletal muscle cell differentiation.

doi: 10.1006/dbio.1997.8527

Figure Lengend Snippet: FIG. 4. The effects of BUdR treatment on levels of b3 integrin FIG. 3. Quantitation of expression of b3 integrin and a5 inte- subunit mRNA and myogenin mRNA in human muscle cells dur- grin subunits in human muscle cells treated with and without ing growth and differentiation. Poly(A)/ mRNA was extracted from BUdR. Homogenates were prepared from human muscle cells cells which had been cultured to confluence (C) in growth medium which had been cultured to subconfluence (SC) and confluence or in growth medium with or without 6.5 mM BUdR and from cells (C) in growth medium with or without 6.5 mM BUdR and from further cultured in fusion medium with or without 6.5 mM BUdR cells further cultured in fusion medium with or without 6.5 mM for 24 hr (24), 48 hr (48), or 96 hr to the appearance of mature BUdR for 24 hr (24), 48 hr (48), and 6 days to the appearance of myotubes (MT) in control cultures (myotubes were not formed in mature myotubes (MT). Western blots were prepared and probed BUdR-treated cultures). Northern blots of the samples were probed for a5 or b3 subunit as described under Materials and Methods. with cDNAs encoding b3 integrin and myogenin as described under Immunoreactive bands were detected by ECL and quantitated Materials and Methods and quantitated using a phosphorimager. by JAVA video analysis, as graphically represented. Units ob- Levels of b3 integrin subunit mRNA and myogenin mRNA are tained by JAVA analysis are arbitrary and represent the pixel expressed relative to measured levels of GAPDH mRNA in the densities of immunoreactive bands as corrected for background. corresponding lane. (A) Cells plated and maintained in the absence (A) b3 integrin. (B) a5 integrin. Symbols: (l) 6.5 mM BUdR; (s) of BUdR. (B) Cells plated and maintained in the presence of 6.5 mM no BUdR. BUdR. Symbols: (s) myogenin; (l) b3 integrin subunit.

Article Snippet: Cells (4 1 105 cells in 1 ml) were added to eppendorf by ECL (a) and quantitated by JAVA video analysis as graphically represented (b).

Techniques: Quantitation Assay, Expressing, Cell Culture, Control, Western Blot, Northern Blot

Journal: Archives of Medical Science : AMS

Article Title: Differential diagnostic CYFRA 21-1 level for benign and malignant pleural effusions: a meta-analysis in the Chinese population

doi: 10.5114/aoms.2012.30831

Figure Lengend Snippet: Description of the included studies

Article Snippet: Jiahe, 2008 [ ] , 56 , ECLA , 20 μg/l , 65 , 90.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Archives of Medical Science : AMS

Article Title: Differential diagnostic CYFRA 21-1 level for benign and malignant pleural effusions: a meta-analysis in the Chinese population

doi: 10.5114/aoms.2012.30831

Figure Lengend Snippet: Summary data from the 17 studies of the utility of CYFRA 21-1 in the differential diagnosis of MPE and BPE

Article Snippet: Jiahe, 2008 [ ] , 56 , ECLA , 20 μg/l , 65 , 90.

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay